In vitro assessment of Bacillus subtilis FJ3 affirms its biocontrol and plant growth promoting potential

Bacillus species and their metabolites have potential alternative uses as chemical pesticides that can limit the growth of plant pathogens enhance crop productivity. The aim this study was to investigate subtilis FJ3 for promoting controlling fungal pathogens. evaluated ability strain promote in vitro by characterizing its growth-promoting traits, which included production hydrolytic enzymes, indole acetic acid, siderophores, biofilm formation, phosphate solubilization. Polymerase Chain Reaction (PCR) testing revealed has produce lipopeptides such fengycin, surfactin, mycosubtilin, pilpastatin. Through antagonism it demonstrated is able inhibit Fusarium oxysporum 52% compared untreated control antagonistic against Aspergillus flavus , niger Rhizopus oryzae using a dual method. minimum inhibitory concentration crude extract resulted 92%, 90%, 81.5%, 56% inhibition A. respectively. In FT-IR GC-MS analysis LPs extract, transmission mass spectrum confirmed existence aforesaid containing β-fatty acids with chain lengths ranging from C14 C21 majority were saturated fatty acids. Greenhouse experimentation significantly diminished disease incidence an average reduction 31.56%. sterilized soil, caused 24.01% 10.46% promotion, respectively, chickpea. results broad-spectrum antifungal applications could be promising candidate development into commercialized biobased product use sustainable agriculture practice.